Knock-out vectors are available for the generation of mutants through intron-retargeting typified by the ClosTron plasmid pMTL007C-E2 [1] – available from the
[1] Kuehne SA, Minton NP. ClosTron-mediated engineering of Clostridium. Bioengineered. 2012; 3(4):247-54.
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