Industry Orders

Tailored Vector Sets

**Tailored vector set as already defined through e-mail correspondence with SBRC.

Modular shuttle plasmid vectors for use in Clostridium bacteria and other Gram-positive anaerobes (Kit 1 & Kit 2), as well as vectors for use in Cupriavidus (Kit 3). Comprising a selection of Gram +ve and -ve replicons, antibiotic markers and various different application specific modules.

Plasmids are sent as lyophilised 50ng/µl DNA ready to be transformed into E. coli.

Kit 1 (Gram +ve)

  • The 18 modules originally described are distributed as part of a kit comprising five plasmids (pMTL80110, pMTL82254, pMTL83353, pMTL84422 & pMTL85141) from which the other 395 possible combinations of modules can be easily constructed in one or a few cloning step(s) using AscI, FseI, PmeI and SbfI.
Order Kit 1 Here

Kit 2 (Gram +ve)

  • Seven plasmids (pMTL89151, pMLT86151, pMTL87151, pMTL81151, pMTL88155, pMTL84131, pMLT82255) comprising five new Gram-positive replicons and the fluorescent marker FAST.
Order Kit 2 Here

Kit 3 (Gram -ve)

  • The 18 modules originally described are distributed as part of a kit comprising six plasmids (pMTL70621, pMTL71302, pMTL72113, pMTL73414, pMTL74131 & pMTL75511) from which all other possible combinations of modules can be easily constructed in one or a few cloning step(s) using AscI, FseI, PmeI and SbfI.
Order Kit 3 Here

Single Modular Vectors for Kit 1

You can order a single modular vector by clicking the relevant button below:

Order pMTL80110
Order pMTL82254
Order pMTL83353
Order pMTL84422
Order pMTL85141

CRISPR CAS-9 Vectors

RiboCas

Supplied as a kit HERE, comprising five vectors (pRECas1, pRECas2, pRECas1-p15a, pRGCas1 & pRGCas2) that incorporate two different riboswitches controlling Cas9 (E&G), and a choice of promoters controlling the expression of the sgRNA, either ParaE (1) or the J23119 promoter (2). Four of the plasmids are based on the ColE1 replicon, while the fifth uses the low copy number p15a replication region.

Order RiboCas Kit

RiboCas Single Modular Vectors

Order pRECas1
Order pRECas2
Order pRECas1-p15a
Order pRGCas1
Order pRGCas2

trCas9 (truncated Cas9)

The five vectors (pMTL431521, pMTL431511, pMTL431211, pMTL432211, pMTL431541) that are supplied as part of the kit, HERE, are all based on the pCB102 Gram +ve replicon, rely on the Pthl promoter for trCas9 expression, carrying the oriT region of RK2, incorporate either ermB or catP as the selectable marker, carry ColE1 or p15a as the Gram -ve replicon and rely on either the ParaE or PJ23119 promoter for expression of the sgRNA.

Order trCas9 Kit

trCas9 Single Modular Vectors

Order pMTL431521
Order pMTL431511
Order pMTL431211
Order pMTL432211
Order pMTL431541

Donor Strains

CA434

= HB101 (R702): F- mcrB mrr hsdS20(rb-mb-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 (SmR) glnV44 λ-)

Order CA434
sExpress

= NEB Express (R702): fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10

Order sExpress
InterStellar

= Stellar (R702): F – ara, Δ(lac-proAB) [Φ80d lacZΔM15], rpsL(str), thi, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, dam, dcm

Order InterStellar
TopSex

= Top10 (R702): mcrA, Δ(mrr-hsdRMS-mcrBC), Phi80lacZ(del)M15, ΔlacX74, deoR, recA1, araD139, Δ(ara-leu)7697, galU, galK, rpsL(SmR), endA1, nupG

Order TopSex
S17.1

= pro, res− hsdR17 (rK− mK+) recA− with an integrated RP4-2-Tc::Mu-Km::Tn7, TpR – λpir

Order S17.1

Queries should be addressed to: vectors@nottingham.ac.uk 

pMTL80000 modular plasmids at ClosTron http://clostron.com/pMTL80000.php