Tailored Vector Sets
**Tailored vector set as already defined through e-mail correspondence with SBRC.
Modular shuttle plasmid vectors for use in Clostridium bacteria and other Gram-positive anaerobes. Comprising a selection of Gram +ve and -ve replicons, antibiotic markers and various different application specific modules.
Plasmids are sent as lyophilised 50ng/µl DNA ready to be transformed into E. coli.
- The 18 modules originally described are distributed as part of a kit comprising five plasmids (pMTL80110, pMTL82254, pMTL83353, pMTL84422 & pMTL85141) from which the other 395 possible combinations of modules can be easily constructed in one or a few cloning step(s) using AscI, FseI, PmeI and SbfI.
- Seven plasmids (pMTL89151, pMLT86151, pMTL87151, pMTL81151, pMTL88155, pMTL84131, pMLT82255) comprising five new Gram-positive replicons and the fluorescent marker FAST.
Single Modular Vectors for Kit 1
You can order a single modular vector by clicking the relevant button below:
CRISPR CAS-9 Vectors
Supplied as a kit HERE, comprising five vectors (pRECas1, pRECas2, pRECas1-p15a, pRGCas1 & pRGCas2) that incorporate two different riboswitches controlling Cas9 (E&G), and a choice of promoters controlling the expression of the sgRNA, either ParaE (1) or the J23119 promoter (2). Four of the plasmids are based on the ColE1 replicon, while the fifth uses the low copy number p15a replication region.
RiboCas Single Modular Vectors
trCas9 (truncated Cas9)
The five vectors (pMTL431521, pMTL431511, pMTL431211, pMTL432211, pMTL431541) that are supplied as part of the kit, HERE, are all based on the pCB102 Gram +ve replicon, rely on the Pthl promoter for trCas9 expression, carrying the oriT region of RK2, incorporate either ermB or catP as the selectable marker, carry ColE1 or p15a as the Gram -ve replicon and rely on either the ParaE or PJ23119 promoter for expression of the sgRNA.
trCas9 Single Modular Vectors
= HB101 (R702): F- mcrB mrr hsdS20(rb-mb-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 (SmR) glnV44 λ-)
= NEB Express (R702): fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10
= Stellar (R702): F – ara, Δ(lac-proAB) [Φ80d lacZΔM15], rpsL(str), thi, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, dam, dcm
= Top10 (R702): mcrA, Δ(mrr-hsdRMS-mcrBC), Phi80lacZ(del)M15, ΔlacX74, deoR, recA1, araD139, Δ(ara-leu)7697, galU, galK, rpsL(SmR), endA1, nupG
Queries should be addressed to: email@example.com
pMTL80000 modular plasmids at ClosTron http://clostron.com/pMTL80000.php